An Efficient, Large-Scale Survey of Hepatitis C Viremia in the Democratic Republic of the Congo Using Dried Blood Spots

Parr, Jonathan B.; Lodge, Evans K.; Holzmayer, Vera; Pepin, Jacques; Frost, Eric H.; Fried, Michael W.; McGivern, David R.; Lemon, Stanley M.; Keeler, Corinna; Emch, Michael E.; Mwandagalirwa, Kashamuka; Tshefu, Antoinette K.; Fwamba, Franck; Muwonga, Jeremie; Meshnick, Steven R.; & Cloherty, Gavin A. (Forthcoming). An Efficient, Large-Scale Survey of Hepatitis C Viremia in the Democratic Republic of the Congo Using Dried Blood Spots. Clinical Infectious Diseases.

Parr, Jonathan B.; Lodge, Evans K.; Holzmayer, Vera; Pepin, Jacques; Frost, Eric H.; Fried, Michael W.; McGivern, David R.; Lemon, Stanley M.; Keeler, Corinna; Emch, Michael E.; Mwandagalirwa, Kashamuka; Tshefu, Antoinette K.; Fwamba, Franck; Muwonga, Jeremie; Meshnick, Steven R.; & Cloherty, Gavin A. (Forthcoming). An Efficient, Large-Scale Survey of Hepatitis C Viremia in the Democratic Republic of the Congo Using Dried Blood Spots. Clinical Infectious Diseases.

Octet Stream icon 10642.ris — Octet Stream, 2 kB (2370 bytes)

Background: Efficient viral load testing is needed for hepatitis C (HCV) surveillance and diagnosis. HCV viral load testing using dried blood spots (DBSs), made with a single drop of finger-prick whole blood on filter paper, is a promising alternative to traditional serum- or plasma-based approaches. Methods: We adapted the Abbott Molecular m2000 instrument for high-throughput HCV viremia testing using DBSs with simple specimen processing and applied these methods to estimate the national burden of infection in the Democratic Republic of the Congo (DRC). We tested DBSs collected during the 2013-2014 DRC Demographic and Health Survey, including 1309 adults >/=40 years of age. HCV-positive samples underwent targeted sequencing, genotyping, and phylogenetic analyses. Results: This high-throughput screening approach reliably identified HCV RNA extracted from DBSs prepared using whole blood, with a 95% limit of detection of 1196 (95% confidence interval [CI], 866-2280) IU/mL for individual 6-mm punches and 494 (95% CI, 372-1228) IU/mL for larger 12-mm punches. Fifteen infections were identified among samples from the DRC Demographic and Health Survey; the weighted country-wide prevalence of HCV viremia was 0.9% (95% CI, 0.3%-1.6%) among adults >/=40 years of age and 0.7% (95% CI, .6%-.8%) among human immunodeficiency virus-infected subjects. All successfully genotyped cases were due to genotype 4 infection. Conclusions: DBS-based HCV testing represents a useful tool for the diagnosis and surveillance of HCV viremia and can easily be incorporated into specimen referral systems. Among adults >/=40 years of age in the DRC, 100000-200000 may have active infection and be eligible for treatment.




JOUR



Parr, Jonathan B.
Lodge, Evans K.
Holzmayer, Vera
Pepin, Jacques
Frost, Eric H.
Fried, Michael W.
McGivern, David R.
Lemon, Stanley M.
Keeler, Corinna
Emch, Michael E.
Mwandagalirwa, Kashamuka
Tshefu, Antoinette K.
Fwamba, Franck
Muwonga, Jeremie
Meshnick, Steven R.
Cloherty, Gavin A.



Forthcoming


Clinical Infectious Diseases













10642

Wink Plone Theme by Quintagroup © 2013.

Personal tools
This is themeComment for Wink theme