Pre-Diagnostic Smoking Is Associated with Binary and Quantitative Measures of ER Protein and ESR1 mRNA Expression in Breast Tumors

Butler, Ebonee N.; Bensen, Jeannette T.; Chen, Mengjie; Conway, Kathleen; Richardson, David B.; Sun, Xuezheng; Geradts, Joseph; Olshan, Andrew F.; & Troester, Melissa A. (Forthcoming). Pre-Diagnostic Smoking Is Associated with Binary and Quantitative Measures of ER Protein and ESR1 mRNA Expression in Breast Tumors. Cancer Epidemiology, Biomarkers & Prevention.

Butler, Ebonee N.; Bensen, Jeannette T.; Chen, Mengjie; Conway, Kathleen; Richardson, David B.; Sun, Xuezheng; Geradts, Joseph; Olshan, Andrew F.; & Troester, Melissa A. (Forthcoming). Pre-Diagnostic Smoking Is Associated with Binary and Quantitative Measures of ER Protein and ESR1 mRNA Expression in Breast Tumors. Cancer Epidemiology, Biomarkers & Prevention.

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Introduction: Smoking is a possible risk factor for breast cancer and has been linked to increased risk of estrogen-receptor positive (ER+) disease in some epidemiologic studies. It is unknown whether smoking has quantitative effects on ER expression. Methods: We examined relationships between smoking and ER expression from tumors of 1,888 women diagnosed with invasive breast cancer from a population-based study in North Carolina. ER expression was characterized using binary (+/-) and continuous measures for ER protein, ESR1 mRNA, and a multigene luminal score (LS) that serves as a measure of estrogen signaling in breast tumors. We used logistic and linear regression models to estimate temporal and dose-dependent associations between smoking and ER measures. Results: The odds of ER+, ESR1+, and LS+ tumors among current smokers (at time of diagnosis), those who smoked 20 or more years, and those who smoked within 5 years of diagnosis were nearly double those of non-smokers. Quantitative levels of ESR1 were highest among current smokers compared to never smokers overall [mean (log2) = 9.2 vs 8.7, p < 0.05] and among ER+ cases; however, we did not observe associations between smoking measures and continuous ER protein expression. Conclusions: In relationship to breast cancer diagnosis, recent smoking was associated with higher odds of the ER+, ESR1+, and LS+ subtype. Current smoking was associated with elevated ESR1 mRNA levels and an elevated luminal score, but not with altered ER protein. Impact A multigene luminal score and single-gene ESR1 mRNA may capture tumor changes associated with smoking.




JOUR



Butler, Ebonee N.
Bensen, Jeannette T.
Chen, Mengjie
Conway, Kathleen
Richardson, David B.
Sun, Xuezheng
Geradts, Joseph
Olshan, Andrew F.
Troester, Melissa A.



Forthcoming


Cancer Epidemiology, Biomarkers & Prevention













10672

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