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Cytomegalovirus Antibodies in Dried Blood Spots: A Minimally Invasive Method for Assessing Stress, Immune Function, and Aging

Citation

Dowd, Jennifer Beam; Aiello, Allison E.; Chyu, Laura; Huang, Yuan-Yen; & McDade, Thomas W. (2011). Cytomegalovirus Antibodies in Dried Blood Spots: A Minimally Invasive Method for Assessing Stress, Immune Function, and Aging. Immunity and Ageing, 8(1), 3. PMCID: PMC3031243

Abstract

BACKGROUND: Cytomegalovirus (CMV) is a prevalent herpesvirus with links to both stress and aging. This paper describes and validates a minimally invasive method for assessing antibodies against CMV in finger stick whole blood spot samples for use as an indirect marker of an aspect of cell-mediated immunity.
RESULTS: Analysis of CMV in dried blood spot samples (DBS) was based on modifications of a commercially available protocol for quantifying CMV antibodies in serum or plasma. The method was evaluated through analysis of precision, reliability, linearity, and correlation between matched serum and DBS samples collected from 75 volunteers. Correlation between DBS and plasma values was linear and high (Pearson correlation R = .96), and precision, reliability, and linearity of the DBS assay were within acceptable ranges.
CONCLUSIONS: The validity of a DBS assay for CMV antibodies will enable its inclusion in population-based surveys and other studies collecting DBS samples in non-clinical settings, increasing scientific understanding of the interaction of social and biological stress and immune function.

URL

http://dx.doi.org/10.1186/1742-4933-8-3

Reference Type

Journal Article

Year Published

2011

Journal Title

Immunity and Ageing

Author(s)

Dowd, Jennifer Beam
Aiello, Allison E.
Chyu, Laura
Huang, Yuan-Yen
McDade, Thomas W.

PMCID

PMC3031243