Citation
Harwood, Valerie J.; Boehm, Alexandria B.; Sassoubre, Lauren M.; Vijayavel, Kannappan; Stewart, Jill R.; Fong, Theng-Theng; Caprais, Marie-Paule; Converse, Reagan R.; Diston, David; & Ebdon, James, et al. (2013). Performance of Viruses and Bacteriophages for Fecal Source Determination in a Multi-Laboratory, Comparative Study. Water Research, 47(18), 6929-6943.Abstract
An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages (FRNAPH) as general indicators of fecal contamination. Bacteriophage methods targeting human fecal sources included genotyping of FRNAPH isolates and plaque formation on bacterial hostsURL
http://dx.doi.org/10.1016/j.watres.2013.04.064Reference Type
Journal ArticleYear Published
2013Journal Title
Water ResearchAuthor(s)
Harwood, Valerie J.Boehm, Alexandria B.
Sassoubre, Lauren M.
Vijayavel, Kannappan
Stewart, Jill R.
Fong, Theng-Theng
Caprais, Marie-Paule
Converse, Reagan R.
Diston, David
Ebdon, James
Fuhrman, Jed A.
Gourmelon, Michele
Gentry-Shields, Jennifer
Griffith, John F.
Kashian, Donna R.
Noble, Rachel T.
Taylor, Huw
Wicki, Melanie