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Citation

Chen, Jane S.; Pettifor, Audrey E.; Nelson, Julie A. E.; Phiri, Sam; Pasquale, Dana K.; Kumwenda, Wiza; Kamanga, Gift; Cottrell, Mackenzie L.; Sykes, Craig; & Dm Kashuba, Angela, et al. (Online ahead of print). Blood and Genital Fluid Viral Load Trajectories among Treated and Untreated Persons with Acute HIV Infection in Malawi. Journal of Acquired Immune Deficiency Syndrome.

Abstract

BACKGROUND: Persons with acute HIV infection (AHI) are highly infectious and responsible for a disproportionate share of incident infections. Immediate antiretroviral therapy (ART) rapidly reduces blood viral loads (VL), but genital VLs after ART initiation during AHI are less well described.
SETTING: Lilongwe, Malawi, 2012-2014.
METHODS: HIV-seronegative and -serodiscordant persons ≥18 years were screened for AHI (RNA positive), and randomized to standard care, behavioral intervention, or behavioral intervention plus short-term ART (raltegravir/emtricitabine/tenofovir) (1:2:2). Persons who were ART eligible under Malawi guidelines could receive first-line therapy. Blood and genital VLs were assessed at weeks 1, 4, 8, and 12. Fisher's Exact test was used to compare viral suppression by ART status.
RESULTS: 46 persons with AHI enrolled, 17 of whom started ART within 12 weeks. Median blood VL at AHI diagnosis was 836,115 copies/mL. At week 12, 7% (1/14) of those who initiated ART had a blood VL ≥400 copies/mL, compared to 100% (23/23; p<0.0001) of those who did not initiate ART (median VL: 61,605 copies/mL). Median genital VL at week 1 was 772 copies/mL, with 13/22 (59%) having VL ≥400 copies/mL. At week 12, 0/10 (0%) of those who initiated ART had genital VL ≥400 copies/mL, compared to 7/15 (47%) of those who did not initiate ART (p=0.02).
CONCLUSION: Although highly correlated, VLs in blood and genital fluids occupy discrete biological compartments with distinct virologic dynamics. Our results corroborate the dramatic reduction in both compartments after ART initiation. Increasing AHI screening and rapidly initiating treatment is key to interrupt transmission.

URL

http://dx.doi.org/10.1097/qai.0000000000002917

Reference Type

Journal Article

Year Published

Online ahead of print

Journal Title

Journal of Acquired Immune Deficiency Syndrome

Author(s)

Chen, Jane S.
Pettifor, Audrey E.
Nelson, Julie A. E.
Phiri, Sam
Pasquale, Dana K.
Kumwenda, Wiza
Kamanga, Gift
Cottrell, Mackenzie L.
Sykes, Craig
Dm Kashuba, Angela
Tegha, Gerald
Krysiak, Robert
Thengolose, Isaac
Cohen, Myron S.
Hoffman, Irving F.
Miller, William C.
Rutstein, Sarah E.

Article Type

Regular

Continent/Country

Malawi

ORCiD

Pettifor - 0000-0002-3387-0817