Menu Close

Impact of Technical Sources of Variation on the Hand Microbiome Dynamics of Healthcare Workers

Citation

Rosenthal, Mariana; Aiello, Allison E.; Chenoweth, Carol; Goldberg, Deborah; Larson, Elaine L.; Gloor, Gregory; & Foxman, Betsy (2014). Impact of Technical Sources of Variation on the Hand Microbiome Dynamics of Healthcare Workers. PLOS ONE, 9(2), e88999. PMCID: PMC3925205

Abstract

We assessed the dynamics of hand microbial community structure of 34 healthcare workers from a single surgical intensive care unit over a short (3 week) time period, whilst taking into account the technical sources of variability introduced by specimen collection, DNA extraction, and sequencing. Sample collection took place at 3 different time points. Only the sampling collection method appeared to have a significant impact on the observed hand microbial community structure among the healthcare workers. Analysis of samples collected using glove-juice showed a slightly more similar microbial composition within individual hand samples over time than between the hands of different individuals over time. This was not true for samples collected using a swab, where samples from a single individual were no more similar to each other over time than those among other individuals over time, suggesting they were essentially independent. DNA extraction techniques (lysozyme only versus enzyme cocktail) and sequencing (replicate set 1 versus 2) using Ion Torrent Personal Genome Machine, were not influential to the microbial community structures. Glove-juice sample collection may likely be the method of choice in hand hygiene studies in the healthcare setting.

URL

http://dx.doi.org/10.1371/journal.pone.0088999

Reference Type

Journal Article

Year Published

2014

Journal Title

PLOS ONE

Author(s)

Rosenthal, Mariana
Aiello, Allison E.
Chenoweth, Carol
Goldberg, Deborah
Larson, Elaine L.
Gloor, Gregory
Foxman, Betsy

PMCID

PMC3925205