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McCullough, Lauren E.; Mendez, Michelle A.; Miller, Erline E.; Murtha, Amy P.; Murphy, Susan K.; & Hoyo, Cathrine (2015). Associations between Prenatal Physical Activity, Birth Weight, and DNA Methylation at Genomically Imprinted Domains in a Multiethnic Newborn Cohort. Epigenetics, 10(7), 597-606. PMCID: PMC4622928


Birth weight is a commonly used indicator of the fetal environment and a predictor of future health outcomes. While the etiology of birth weight extremes is likely multifactorial, epidemiologic data suggest that prenatal physical activity (PA) may play an important role. The mechanisms underlying this association remain unresolved, although epigenetics has been proposed. This study aimed to estimate associations between prenatal PA, birth weight, and newborn DNA methylation levels at differentially methylated regions (DMRs) regulating four imprinted genes known to be important in fetal development. Study participants (N = 1281) were enrolled as part of the Newborn Epigenetics Study. Prenatal PA was ascertained using the Pregnancy Physical Activity Questionnaire, and birth weight data obtained from hospital records. Among 484 term mother-infant pairs, imprinted gene methylation levels were measured at DMRs using bisulfite pyrosequencing. Generalized linear and logistic regression models were used to estimate associations. After adjusting for preterm birth and race/ethnicity, we found that infants born to mothers in the highest quartile of total non-sedentary time had lower birth weight compared to infants of mothers in the lowest quartile (


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McCullough, Lauren E.
Mendez, Michelle A.
Miller, Erline E.
Murtha, Amy P.
Murphy, Susan K.
Hoyo, Cathrine