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F+ RNA Coliphage Typing for Microbial Source Tracking in Surface Waters

Citation

Stewart-Pullaro, Jill R.; Daugomah, James W.; Chestnut, David E.; Graves, David A.; Sobsey, Mark D.; & Scott, Geoffrey I. (2006). F+ RNA Coliphage Typing for Microbial Source Tracking in Surface Waters. Journal of Applied Microbiology, 101(5), 1015-1026.

Abstract

AIMS: The utility of coliphages to detect and track faecal pollution was evaluated using South Carolina surface waters that exceeded State faecal coliform standards.
METHODS AND RESULTS: Coliphages were isolated from 117 surface water samples by single agar layer (SAL) and enrichment presence/absence (EP/A) methods. Confirmed F+ RNA coliphages were typed for microbial source tracking using a library-independent approach. Concentrations of somatic coliphages using 37 and 44.5 degrees C incubation temperatures were found to be significantly different and the higher temperature may be more specific for faecal contamination. The EP/A technique detected coliphages infecting

URL

http://dx.doi.org/10.1111/j.1365-2672.2006.03011.x

Reference Type

Journal Article

Year Published

2006

Journal Title

Journal of Applied Microbiology

Author(s)

Stewart-Pullaro, Jill R.
Daugomah, James W.
Chestnut, David E.
Graves, David A.
Sobsey, Mark D.
Scott, Geoffrey I.