Citation
Bam, Marpe; Yang, Xiaoming; Busbee, Brandon P.; Aiello, Allison E.; Uddin, Monica; Ginsberg, Jay P.; Galea, Sandro; Nagarkatti, Prakash S.; & Nagarkatti, Mitzi (2020). Increased H3K4me3 Methylation and Decreased miR-7113-5p Expression Lead to Enhanced Wnt/β-Catenin Signaling in Immune Cells from PTSD Patients Leading to Inflammatory Phenotype. Molecular Medicine, 26(1), 110. PMCID: PMC7666486Abstract
BACKGROUND: Posttraumatic stress disorder (PTSD) is a psychiatric disorder accompanied by chronic peripheral inflammation. What triggers inflammation in PTSD is currently unclear. In the present study, we identified potential defects in signaling pathways in peripheral blood mononuclear cells (PBMCs) from individuals with PTSD.METHODS: RNAseq (5 samples each for controls and PTSD), ChIPseq (5 samples each) and miRNA array (6 samples each) were used in combination with bioinformatics tools to identify dysregulated genes in PBMCs. Real time qRT-PCR (24 samples each) and in vitro assays were employed to validate our primary findings and hypothesis.
RESULTS: By RNA-seq analysis of PBMCs, we found that Wnt signaling pathway was upregulated in PTSD when compared to normal controls. Specifically, we found increased expression of WNT10B in the PTSD group when compared to controls. Our findings were confirmed using NCBI's GEO database involving a larger sample size. Additionally, in vitro activation studies revealed that activated but not naïve PBMCs from control individuals expressed more IFN
URL
http://dx.doi.org/10.1186/s10020-020-00238-3Reference Type
Journal ArticleYear Published
2020Journal Title
Molecular MedicineAuthor(s)
Bam, MarpeYang, Xiaoming
Busbee, Brandon P.
Aiello, Allison E.
Uddin, Monica
Ginsberg, Jay P.
Galea, Sandro
Nagarkatti, Prakash S.
Nagarkatti, Mitzi